ObjectiveTo realize the species and antimicrobial resistance of pathogens isolated from blood culture in recent 3 years at a hospital, and provide reference for the rational use of antimicrobial agents.MethodsBlood culture results of 3 880 clinical specimens detected from January 2009 to December 2011 were analyzed using WHONET 5.5 software.ResultsOf all blood specimens, 410(10.57%) were isolated bacteria, gramnegative bacilli, grampositive cocci, grampositive bacilli, anaerobic bacteria and fungi were 210 isolates（50.48%），128（30.77%），15（3.60%），17（4.09%） and 46（11.05%）,respectively. The top five pathogens were Escherichia coli (88 isolates, 21.15%), Staphylococcus aureus(36,8.66%),Staphylococcus epidermidis(28, 6.73%), Klebsiella pneumoniae (26, 6.25%)， and Candida parapsilosis(27,6.49%). The positive rate of extendedspectrum βlactamaseproducing Escherichia coli and Klebsiella pneumoniae was 60.70% and 28.60% respectively, all strains were sensitive to imipenem and meropenem; the positive rate of methicillinresistant Staphylococcus aureus and methicillinresistant Staphylococcus epidermidis was 25.00% and 100.00% respectively; the sensitive rates of Candida parapsilosis to 5fluorocytosine, amphotericin B, fluconazole and itraconazole were all 100%.ConclusionEnterobacteriaceae are the main pathogens in blood culture in this hospital, the next is Staphylococcus spp., infection rate of fungus is also high; the change in pathogen in blood culture and antimicrobial resistance should be paid attention, and healthcareassociated bloodstream infection should be prevented and controlled.