ObjectiveTo investigate the distribution and antimicrobial resistance of pathogens isolated from blood culture from a hospital.MethodsData of pathogens isolated from blood specimens between 2008 and 2011 were analyzed retrospectively.ResultsA total of 285 pathogenic isolates were obtained from 1 780 specimens, positive rate was 16.01%. The number of grampositive cocci were 155 isolates（54.39%）,the major were Staphylococcus aureus （84 isolates，29.48%）and coagulase negative Staphylococcus （38 isolates，12.28%）; the number of gramnegative bacilli were 103 isolates(36.14%),the major were Escherichia coli（43 isolates，15.09%）,Pseudomonas aeruginosa（19 isolates，6.67%）,and Klebsiella pneumoniae（13 isolates，4.56%）;the number of fungi was 27 isolates (9.47%),the major was Candida albicans(13 isolates,4.56%).The resistant rate of grampositive cocci was high, but all strains were sensitive to vancomycin and teicoplanin, 40.48% of Staphylococcus aureus and 57.14% of coagulase negative Staphylococcus were methicillinresistant. Gramnegative bacilli were sensitive to imipenem, meropenem,and enzyme inhibitorcontaining antimicrobial drugs; 31 extendedspectrum βlactamaseproducing isolates were detected, 25（58.14%） of which were Escherichia coli， 6（46.15%）were Klebsiella pneumoniae.ConclusionThe major pathogens isolated from blood culture from this hospital are grampositive bacteria, and antimicrobial resistance is high, clinicians should choose antimicrobial agents according to antimicrobial susceptibility testing results, so as to reduce the emergence of resistant strains.