ObjectiveTo realize the clinical distribution and antimicrobial resistance of common nonfermentative bacteria from a hospital.MethodsData of nonfermentative bacteria isolated between January 1, 2012 and December 31,2012 were analyzed retrospectively.ResultsA total of 427 （9.37%）isolates of nonfermentative bacteria were isolated from 4 559 specimens，405 (94.85%,405/427) isolates of which were Pseudomonas aeruginosa, Acinetobacter baumannii and Stenotrophomonas maltophilia, these strains were mainly isolated from sputum specimens（75.89%-78.79%） and with high antimicrobial resistance, but the resistant rate of Pseudomonas aeruginosa to amikacin, meropenem, and polymyxin B was low(14.54%,11.89%, and 14.98% respectively), the resistant rate of Acinetobacter baumannii to amikacin, meropenem, and polymyxin B was low(6.25%,6.25%, and 8.04% respectively), the resistant rate of Stenotrophomonas maltophilia to compound sulfamethoxazole and polymyxin B was low(3.03% and 13.64% respectively).ConclusionNonfermentative bacteria in this hospital are mainly isolated from lower respiratory tract specimens, and antimicrobial resistance is high, antimicrobial agents should be used according to antimicrobial susceptibility testing result, and spread of infection should be prevented.