ObjectiveTo investigate the distribution and antimicrobial resistance of pathogens from blood culture, and to guide the rational use of antimicrobial agents.MethodsData of pathogens from blood specimens of inpatients and outpatients in a hospital between January 2008 and December 2011were analyzed statistically.ResultsOf 670 pathogenic isolates from blood culture, gramnegative bacilli, grampositive cocci and fungi accounted for 45.67%(n=306),49.11% (n=329) and 5.22%(n=35) respectively; the top 3 were Coagulase negative staphylococcus(23.88%), Escherichia coli(19.40%) and Staphylococcus aureus(12.98%).Susceptibility of multidrugresistant Staphylococcus to compound sulfamethoxazole, linezolid and vancomycin were relatively high (resistant rate were 0-20.34%).The resistant rates of gramnegative bacilli (except Acinetobacter spp.) to cefoperazone/sulbactam,piperacillin/ tazobactam and amikacin were 0-27.27%. Acinetobacter spp. had the highest resistant rate. The most common fungus in blood culture were Candida parapsilosis (2.38%) and Candida albicans(1.79%).ConclusionEarly blood culture and monitor on multidrugresistant pathogenic isolates can provide reliable evidence for clinical diagnosis and treatment.