ObjectiveTo investigate clinical distribution and antimicrobial resistance of multidrugresistant organisms(MDROs)in a hospital in 2012, and provide reference for the prevention of MDROs infection.MethodsDistribution and antimicrobial susceptibility testing results of MDROs isolated from patients in a hospital between January and December 2012 were analyzed by targeted monitoring method. ResultsA total of 370 MDROs strains were isolated, isolation rate of gramnegative bacilli and grampositive cocci was 92.16%(n=341) and 7.84%(n=29,all were methicillinresistant Staphylococcus aureus［MRSA］)respectively; the top five departments with high MDROs isolation rates were intensive care unit(n=79 isolates), department of neurosurgery (n=65), neurology (n=40) general surgery(n=39), and geriatrics(n=32). Resistant rates of 29 MRSA strains to compound sulfamethoxazole,tigecycline,and rifampicin were all>60%, no vancomycinresistant strain was detected; resistant rates of extendedspectrum betalactamase (ESBLs)producing Escherichia coli and Klebsiella pneumoniae to imipenem were >75%,resistant rates to cefoperazone / sulbactam and amikacin were about 50%; multidrugresistant Pseudomonas aeruginosa and Acinetobacter baumannii were generally not sensitive to antimicrobial agents, multidrugresistant Acinetobacter baumannii were almost pandrugresistant strains.ConclusionThe major MDROs isolated from this hospital are gramnegative bacteria, strains are mainly from patients with severe diseases and elderly age. Monitor and control measures on healthcareassociated infection should be intensified to prevent the spread of MDROs.