ObjectiveTo compare the cleaning efficacy of gynecological and obstetrical surgical instruments by different cleaning methods, so as to find out the effective cleaning method.MethodsSixty sets of artificial abortion instruments used in an outpatient department of gynecology and obstetrics were randomly divided into 3 groups(A,B,C, 20 for each), group A adopted multienzyme immersion in combination with manual clean, group B adopted multienzyme immersion combined with machine wash, and group C adopted ultrasonic clean with machine wash, cleaning efficacy of three groups were compared. ResultsAssessment by visual inspection and magnifying glass showed that the qualified rate of cleaning of group C was significantly higher than group A and B(99.78% vs 73.48%; 99.78% vs 78.70%; both＜0.0125）;group A and B was not significantly different (P＞0.0125). The qualified rate of cleaning of nonlumen instruments in group A and B were both significantly higher than lumen instruments (both P<0.05). Occult blood test result showed that the qualified rate of group C was significantly higher than group A and B (99.16% vs 75.00%; 99.16% vs 80.61%; both P＜0.0125）; group A and B was not significantly different (P＞0.0125). The qualified rate of cleaning between lumen and nonlumen instruments of three groups was not significantly different respectively (all P>0.05).ConclusionOn the basis of immersion in multienzyme,ultrasonic clean in combination with machine wash can improve the cleaning quality of intricate and lumen instruments and ensure the sterilization efficacy.