ObjectiveTo realize the current status of central sterile supply departments(CSSDs) in hospitals, and provide the basis for making improvement measures.MethodsField investigation was adopted to study the resource distribution, personnel structure, and the quality of medical instrument cleaning in 78 hospitals in Chongqing.ResultsQualified results of 78 hospitals was 24.36%(19/78),basically qualified rate 52.56%(41/78), unqualified rate 23.08%(18/78)；inspection results of different types of hospitals were significantly different（Hc=16.643，P=0.002）, qualified hospitals mainly concentrated on city and countylevel hospitals.Unqualified rate of CSSD construction area was 78.21%（n=61）, unqualified rate of personal allocation was 75.64%（n=59）,concentrated management of operating rooms and CSSDs was 52.56%（n=41）, mainly concentrated on city and countylevel hospitals; decentralized management accounted for 47.44%（n=37）,58（74.36%）hospitals used repeated cleaning stainless steel tables for receiving, 50% didn’t install automated cleaning machine and drying cabinet, 42.31%（33/78）performed manual cleaning，40（51.28%）didn’t equip with water treatment system;12（15.38%）hospitals used patched or worn clothing for packing, 47 didn’t equip with hard metal container, 37（47.44%）had no heat sealing machine, 21(26.92%) had no detection equipment for cleaning quality, 41（52.56%）had no low temperature sterilization instruments;52（66.67%）hospitals performed biological monitoring on high pressure steam sterilizer.ConclusionClean and disinfection equipments in CSSDs in city and countylevel hospitals are wellappointed, procedures of clean and disinfection are standard, and can achieve the standard of clean quality; while hospitals affiliated to factories and private hospitals are not wellappointed, procedures of clean and disinfection are not standard. Construction of CSSD should be standard,so as to ensure the effectiveness of clean, disinfection and sterilization of surgical instruments.