ObjectiveTo investigate the detection results of HBsAg, antiHCV, antiTreponema pallidum antibody (antiTP)and antiHIV in patients before transfusion and surgery.MethodsFour infection indicators of 28 165 patients were detected by enzymelinked immunosorbent assay from June 2011 to May 2012,results were analyzed statistically.ResultsOf 28 165 patients, total positive rate was 12.15% （n=3 422）,the positive rate of HBsAg, antiHCV, antiTP and antiHIV was 8.69%（n=2 447）,1.31%（n=368）,2.07%（n=583）,and 0.09%(n=24) respectively. The positive rate of HBsAg, antiHCV and antiHIV in male was higher than female (χ2 was 36.64, 28.95,and 4.82，respectively,all P＜0.05）.In different age groups,positive rate of all indicators in <20 age group was lowest,while positive rates of HBsAg, antiHCV, and antiHIV were highest in 20-39 and 40-59 age groups,antiTP was highest in ≥60 age group.ConclusionDetection of bloodborne pathogens before transfusion and surgery is helpful for realizing infection status of patients before transfusion and surgery.