ObjectiveTo investigate the prevalence of antiHCV positive among volunteer blood donors in Zhoukou city. MethodsA total of 200 950 blood specimens from voluntary blood donors in Zhoukou from 2007 to 2012 were detected for antiHCV by doubleantibody sandwich enzymelinked immunosorbent assay, results were analyzed. ResultsThe total positive rate of antiHCV was 0.21%（416／200 950）, there were significant difference in positive rates of antiHCV among populations of different genders, ages and educational background（χ2=24.01, 142.31,45.8，respectively,all P<0.01）, the positive rate of antiHCV in female was higher than that of male (［0.27%,205/75 658］ vs ［0.17%, 211/125 292］）; the positive rate of antiHCV was the lowest in donors aged 21-30 years (0.06%,38/63 719）, and highest in donors aged >40 years (0.33%,162/48 871）; the lower donors received education, the higher positive rate of antiHCV they had, the positive rate of antiHCV in donors with junior high school education was 0.26%（107/41 154）. The positive rate of antiHCV in blood donors decreased gradually from 2007 to 2012（χ2=14.576，P<0.05）.ConclusionThe positive rate of antiHCV is low in voluntary blood donors in Zhoukou and is significantly different among different populations.