ObjectiveTo evaluate the effect of antimicrobial management guideline on the daily antimicrobial use rate in a general hospital.MethodsAn oneday pointprevalence survey on antimicrobial use in hospitalized patients was conducted in 2010, 2011,and 2012 by means of crosssectional investigation method.ResultsThe daily utilization rate of antimicrobial agents in 2010, 2011,and 2012 was 65.40%,42.09% and 26.74% respectively，the rate of the submission of specimens for bacterial culture was 22.53%, 35.74% and 41.74% respectively（χ2trend=266.42,29.02，respectively， both P＜0.01）. The utilization rate of single drug was 83.72%,80.27% and 93.41% respectively. the utilization rate of both twodrug and more than threedrug combination kept a downward tendency. The utilization rate of antimicrobial agents in several departments(digestive internal medicine,cardiovascular, hematological tumor,neurology, general surgery,orthopedics, obstetrics, pediatrics,and ophthalmology) showed a decreasing tendency(all P<0.01).ConclusionThe implementation of antimicrobial management guideline has remarkably reduced the daily antimicrobial use rate, and increased etiological detection rate for therapeutic antimicrobial use.