ObjectiveTo realize the characteristics of clinical distribution of multidrugresistant organisms (MDRO) in a hospital,and take specific measures for the prevention and control of infection.MethodsSurveillance data of 891 MDR isolates detected in a hospital between January 1 and December 31, 2012 were analyzed retrospectively.ResultsOf 891 MDR isolates, extendedspectrum βlactamase(ESBL)producing Escherichia coli ranked first（342，38.39%）, followed by ESBLproducing Klebsiella pneumoniae（195，21.89%）,MDR Acinetobacter baumannii（185，20.76%）, methicillinresistant Staphylococcus aureus（138，15.49%）, MDR Pseudomonas aeruginosa（27，3.03%）, ESBLproducing Proteus mirabilis（2，0.22%）,and ESBLproducing Klebsiella oxytoca（2，0.22%）; Bacteria mainly concentrated on general intensive care unit(ICU)(163,18.29%）, department of neurology（136,15.26%），general surgery(103,11.56%),neurosurgery（85,9.54%）, and respiratory diseases department（71,7.97%）.The most common bacteria isolated from sputum was MDR Acinetobacter baumannii (242，50.63%),and mainly concentrated on general ICU; the most common bacteria isolated from urine was ESBLproducing Escherichia coli(141，80.57%), mainly concentrated on the departments of neurology and urology. ConclusionMDRO infection in this hospital mainly concentrate on lower respiratory tract and urinary system. Monitor on highrisk departments and vulnerable patients should be intensified, targeted preventive measures should be stressed to curb MDRO infection and spread.