ObjectiveTo explore the effective measures to improve hand hygiene compliance among health care workers(HCWs) in a hospital.MethodsIn 2012,comprehensive management measures were taken to intervene hand hygiene compliance among HCWs in a hospital.ResultsA total of 72 times of inspection of hand hygiene among HCWs in the whole hospital were conducted, 86 nonhandtouching water taps were transformed, 420 posters pictures and hand washing flow charts were put up. Hand hygiene compliance rate in 2012 was higher than in 2011(60.48% vs 34.50%)（χ2=458.70，P＜0.05）；qualified rate of hand hygiene was higher than in 2011 (82.13% vs 60.04%)(χ2=188.41，P＜0.05）.The consumption of fastacting hand disinfectant of whole hospital and intensive care unit was significantly higher than in 2011(3.15mL /bed/day vs 0.39mL/bed/day,t=2.59; 21.82 mL/bed/day vs 17.81mL/bed/day,t=2.76，both P＜0.05）.ConclusionComprehensive management measures can effectively improve hand hygiene compliance among HCWs.