临床分离肠杆菌科细菌金属β内酰胺酶检测
作者:
作者单位:

作者简介:

黄文祥

通讯作者:

中图分类号:

R378

基金项目:


Detection of metalloβlactamases in clinical isolates of Enterobacteriaceae
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 文章评论
    摘要:

    目的研究某院2007—2011年临床分离的肠杆菌科细菌产金属β内酰胺酶主要基因型及流行情况。方法常规分离菌株,采用纸片扩散法和琼脂稀释法进行药物敏感性试验,改良Hodge试验和Etest MBL确认纸条法表型筛查金属β内酰胺酶,聚合酶链反应(PCR)扩增金属β内酰胺酶基因型,接合试验分析质粒携带基因的转移,脉冲场凝胶电泳(PFGE)分析产金属酶细菌的同源性。结果3 297株肠杆菌科细菌中,对碳青霉烯类抗生素耐药的菌株共41株,其中14株碳青霉烯酶表型阳性,3株肺炎克雷伯菌产IMP8型金属酶,属于同一克隆,并可通过质粒传递。 结论 该院发现3株产IMP8型金属酶的肺炎克雷伯菌,且具有较高的同源性。

    Abstract:

    ObjectiveTo investigate the major genotypes and prevalence of metalloβlactamases (MBLs) in Enterobacteriaceae from a hospital in 2007-2011. MethodsAll strains of Enterobacteriaceae were isolated from clinical specimens by routine method, antimicrobial susceptibility testing was performed by disc diffusion method(KirbyBauer) and agar dilution method, MBLs was screened by both modified Hodge test and Etest  , MBLs genes were amplified by polymerase chain reaction (PCR), the transference of IMP8encoding plasmid was performed by conjugation test , the homology of  MBLsproducing strains was detected by pulsedfield gel electrophoresis (PFGE). ResultsOf 3 297 Enterobacteriaceae isolates ,41 were   resistant to carbapenems, and phenotype confirmation test showed 14 strains produced carbapenemase, 3 Klebsiella pneumoniae isolates carrying blaIMP8 belonged to the same clone, which can be transferred to others by plasmid.ConclusionThree blaIMP8carrying Klebsiella pneumoniae isolates were found in this hospital,and has a high degree of  homology.

    参考文献
    相似文献
引用本文

孙秋,黄文祥,史芳静,等.临床分离肠杆菌科细菌金属β内酰胺酶检测[J]. 中国感染控制杂志,2014,13(6):321-326. DOI:10.3969/j. issn.1671-9638.2014.06.001.
SUN Qiu, HUANG Wenxiang, SHI Fangjing, et al. Detection of metalloβlactamases in clinical isolates of Enterobacteriaceae[J]. Chin J Infect Control, 2014,13(6):321-326. DOI:10.3969/j. issn.1671-9638.2014.06.001.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2013-12-09
  • 最后修改日期:2014-02-12
  • 录用日期:
  • 在线发布日期: 2014-06-30
  • 出版日期: