ObjectiveTo monitor Staphylococcus infection in a hospital, and trace the source of  Staphylococcus isolated from infectious woundrelated hospital environment by molecular analysis. MethodsBy combination of fluorescence quantitative polymerase chain reaction (FQPCR) and culture method, environmental specimens related to 5 patients with wound infection were taken and performed multilocus sequence  and MecA gene typing analysis. ResultsA total of 71 environmental specimens were taken, Staphylococcus accounted for 36.62%（n=26），88.46% (23/26 ) of which were MecA+ methicillinresistant strains. 77.78% （7/9）of  Staphylococcus aureus (S. aureus) and 95.00%（19/20）of coagulase negative Staphylococcus (CNS) were  methicillinresistant strains. Multilocus sequence analysis revealed that ST239 (n=6) was the most common sequence type in S. aureus；Staphylococcal cassette chromosome mec (SCCmec)   analysis showed that the major type of S. aureus was type Ⅲ, and CNS were type Ⅲ and Ⅳ. ConclusionStaphylococcus is common in healthcareassociated infection, and most Staphylococcus are multidrugresistant, continuous monitor on drugresistant Staphylococcus is necessary, and risk of Staphylococcus variant to medical institutes need to be paid attention.