ObjectiveTo evaluate the effect of comprehensive intervention measures on reducing the bacterial colonization of the intensive care unit (ICU) bedside notebook computer keyboards and mice.MethodsAccording to bacterial contamination of ICU bedside notebook computer keyboards and mice, comprehensive intervention measures were taken (including wiping computer keyboards and mice with alcohol twice a day, intensifying hand hygiene, and monitoring handwashing compliance), specimens of keyboards and mice were taken, bacterial detection results were analyzed. ResultsBefore intervention, bacterial counts of keyboards and mice of 8 specimens were all ＞5 CFU/cm2,detection rate of Bacillus subtilis was 64.71%，Micrococcus, coagulase negative staphylococcus, Acinetobacter baumannii, and Klebsiella pneumoniae were 17.65%-58.82%, Escherichia coli and Acinetobacter calcoaceticus were both 5.88%. After comprehensive intervention measures were taken, bacterial counts of only two specimens of keyboards and mice were ＞5 CFU/cm2,the qualified rate of monitoring result after intervention was significantly higher than before intervention (88.24% vs 52.94%; χ2=5.10，P＜0.05）.ConclusionComprehensive intervention measures can significantly reduce bacterial count on ICU bedside notebook computer keyboards and mice, and decrease the occurrence of healthcareassociated infection.