ObjectiveTo monitor the quality of inuse disinfectant,evaluate the causes of exceeding of bacterial content standard, and put forward  the corresponding management measures.  MethodsAccording to the different management methods, disinfectant specimens were divided into experimental group(n=210, from August 2012 to July 2013) and control group (n=190, from August 2011 to July 2012); inuse disinfectant in experimental group was contained with groundglass stoppered bottles, inuse disinfectant in control group was contained with stainless bottles and groundglass stoppered bottles, biological monitor results of two groups were compared. ResultsQualified rate of biological monitor result in experimental group was 100.00%（201/201）,and in control group was 90.00%（171/190）, there was significant difference between two groups（χ2=21.13，P＜0.05）.ConclusionImproving management method can increase the qualified rate of biological monitoring result of inuse disinfectant, and ensure the safety and effectiveness of disinfection.