ObjectiveTo investigate the occurrence of healthcareassociated infection(HAI) and catheterrelated infection in a general intensive care unit(GICU) ,so as to strengthen the management. MethodsTargeted monitor method was adopted, monitor scheme was established , personal monitor contents and forms were designed, hospitalized GICU patients were investigated prospectively between May 2012 and May 2013, and surveyed data were analyzed.ResultsA total of 868 patients were investigated, 65 times (7.49%) of HAI occurred; infection rate per day was 21.49‰, adjust infection rate per day was 7.26‰. Infection rate of ventilatorassociated pneumonia , catheterassociated urinary tract infection ,and central venous catheterrelated bloodstream infection per day was 23.91‰, 3.46‰, and 6.12‰, respectively; the main infection site was respiratory tract ( 67.69%). Sixtyfive isolates of pathogens were detected, 81.54%（n=53）of pathogens were gramnegative bacilli, and with multidrug resistance.ConclusionHAI rate is high in GICU , the use of various types of catheters is an important factor for HAI in GICU patients. Targeted monitoring on ICU is a scientific and effective method , it can timely reflect the weak links of infection control practice and promote HAI control .