ObjectiveTo compare the bacteriostasis effect of two disinfectants on frequently touched object surfaces in laminar flow general intensive care unit (GICU), and investigate bacterial contamination on the object surfaces, so as to provide reference for proper disinfection method and control of infection. MethodsSpecimens from surfaces of bed rails and bedside tables were taken for bacterial culture before being disinfected. Then object surfaces around bed units were disinfected with disinfectant containing 500 mg/L of available chloride (routing group) and GammaTM disinfecting wet wipes (control group)respectively, bacteriostasis rate and qualified rate of bacterial count on object surfaces between two kinds of disinfection methods were compared. ResultsBefore routine disinfection, a total of 87  pathogens were isolated from 200 specimens of object surfaces,bacterial contamination rate was 43.50%. Detection rate of  grampositive bacteria was 78.16%% (n=68),the major were Corynebacterium (26.47%,n=18), Staphylococcus aureus (23.53%,n=16) and  Staphylococcus epidermidis (23.53%,n=16）; detection rate of  gramnegative bacteria was 21.84%(n=19),the major was Acinetobacter baumannii (63.16%,n=12）. After a 10minute disinfection on surfaces, bacteriostasis rate of routine group and control group was （94.89±7.72）% and（96.33±12.88）% respectively，there was  no significant difference between two groups(P>0.05); qualified rates of bacterial count of object surfaces of two groups were both 100%. ConclusionRegular disinfection of object surfaces around bed units, standardization of the manipulation  and hand hygiene compliance of  medical personnels are simple and effective method of cutting off bacteria dissemination and preventing healthcareassociated infection.