ObjectiveTo explore the distribution and drug resistance of pathogens causing bloodstream infection in patients in a general intensive care unit (GICU), and provide reference for the prevention of bloodstream infection and rational use of antimicrobial agents. MethodsFrom January 2011 to December 2013, clinical data of patients who were diagnosed with bloodstream infection were reviewed retrospectively, detected pathogens and drug resistance were analyzed statistically. ResultsThe major pathogens isolated from 385 patients with positive blood culture were  gramnegative bacilli, which accounting for 62.34%; isolation rate of  grampositive cocci and  fungi  was  27.01% and  10.65% respectively. The top five pathogens were Escherichia coli (18.18%), Pseudomonas aeruginosa(16.10%), Staphylococcus aureus (15.59%), Acinetobacter baumannii (13.25%), and Klebsiella pneumoniae (9.09%).The detection rate of methicillinresistant Staphylococcus aureus and methicillinresistant coagulase negative Staphylococcus was 72.55% and 68.34% respectively. Gramnegative bacilli was most sensitive to imipenem and amikacin (resistant rate was 0-35.65%）.ConclusionGramnegative bacilli are the main pathogens in blood culture from GICU in this hospital, and drugresistant rates are high. It’s important to strengthen blood culture of patients with suspected septicemia,  use antimicrobial agents rationally and control infection effectively.