ObjectiveTo improve hand hygiene compliance among health care workers(HCWs) through continuous quality improvement, and effectively reduce the incidence of healthcareassociated infection(HAI). MethodsContinuous quality improvement was performed by adopting plandocheckaction(PDCA) cycle, all HCWs were trained, hand hygiene was stressed, periodical  and random checking was conducted. ResultsAfter the implementation of PDCA cycle, the acknowledge  rate of hand hygiene enhanced from 48.00% to 63.99%; hand hygiene compliance rate enhanced from 65.11％ to 82.40％，the difference were both significant（χ2=12.75, 259.65， respectively， both P＜0.05）.The daily consumption of instant hand antiseptic per 1 000 bed day increased obviously, which was 2.95fold of preimplementation. ConclusionContinuous quality improvement through PDCA cycle can effectively improve hand hygiene compliance rate of HCWs.