ObjectiveTo investigate the qualified rate of midwives’ surgical hand disinfection and perineal incisional infection in parturients, so as to provide reference for reducing perineal incisional infection. MethodsData of 7 148 women who delivered naturally in an obstetrics department between January 2012 and December 2013 were collected, 3 569 midwives who weren’t intervened in surgical hand disinfection between January and December 2012 were as control group, and 3 579 midwives who were intervened between January and December 2013 were as intervention group, the perineal incisional infection rate of parturients and qualified rate of midwives’  hand hygiene of two groups were compared. ResultsThere were no significant difference in maternal age, body mass index, underlying diseases, No. of  vaginal delivery examination, delivery season, delivery lasting time, incision suture technique, antimicrobial prophylaxis, and neonatal weight between intervention group and control group (all P>0.05). The qualified rate of midwives’ surgical hand disinfection method increased from 19.31%（n=689）before intervention to 71.03%（n=2 542） after intervention, the difference was significant between two groups（χ2=752.58，P＜0.05）. Perineal incisional infection rate in intervention group was significantly lower than control group (［(0.28%,10/3 579］ vs ［0.81%， 29/3 569］）, there  was significant difference between two groups （χ2=9.36，P＜0.05）. ConclusionThe improvement of qualified rate of midwives’ hand hygiene can reduce perineal incisional infection rate of parturients.