ObjectiveTo realize the current situation of glove perforation during surgical operation process, and provide basis for preventing surgical glove perforation. MethodsIn January 2014, an investigation on glove perforation was conducted among operation personnel in 9 operating rooms in a hospital, glove perforation rates, sites, causes and noticed ways were analyzed. ResultsA total of 2 909  persontime was investigated, 147 persontime occurred glove perforation， persontime rate of glove perforation was 5.05%; 153 of 5 818 gloves (2.63%) were perforated， 6 persontime occurred perforation of double gloves; gloves perforation rates of each specialized surgery were significantly different (χ2=87.945，P＜0.001）,cardiac surgery（11.84%） and plastic surgery（10.78%）had the hight perforation rates. The common sites of perforation were index finger,thumb, and middle finger,the main cause of perforation was sharp damage by stitches and devices.  Most perforations were noticed intraoperatively, and seldom found postoperatively by naked eyes; gloves of different brands  had different perforation rates（χ2=33.845，P＜0.001）.ConclusionIn order to reduce and prevent the perforation during operation process, glove brands with good quality should be chosen, and measures for the prevention of glove perforation should be formulated according to the types of surgery.