ObjectiveTo compare disinfection efficacy of three different air disinfection methods on the air in blood collection room. MethodsAir disinfection  in blood collection room was conducted by ultraviolet (UV) light, ozone disinfector (OD)and air purification disinfector  respectively , natural sedimentation method was used to take air specimens  before , after disinfection , and during working condition of staff , air disinfection efficacy was detected. ResultsAll three methods could decrease air bacterial count after disinfection , the total number of bacteria in indoor air after disinfection met the class Ⅲ environmental requirement. Under working condition of staff ，the number of bacteria of OD and UV light group increased rapidly, at 4 hours was （7.53±1.28）CFU/5 min·Φ90petri dish and （8.16±1.57）CFU/5 min·Φ90petri dish respectively; air purification disinfector disinfected for 4 hours under the condition of  movement of personnel in blood collection environment, bacteria count still remained ＜4.0 CFU/5 min·Φ90petri dish. ConclusionDisinfecting continuously by air purification disinfector can ensure the effectiveness of air cleanliness in blood collection room.