ObjectiveTo explore the effect of bundle hand hygiene intervention in controlling  healthcareassociated infection(HAI) in a primary comprehensive hospital, so as to improve hand hygiene compliance and correct rate,  and reduce AHI rate. MethodsIn JanuaryJune 2014, bundle hand hygiene intervention among health care workers (HCWs) in a hospital was performed, hand hygiene knowledge awareness rate, hand hygiene compliance and correct rate, hand sanitizer usage and HAI rate before and after intervention were compared. ResultsAfter performing intervention for six months, the awareness rate of hand hygiene knowledge(concept, significance, indications, methods, sanitizer use) of HCWs improved compared with before intervention (P＜0.05);hand hygiene compliance and correct rate were significantly higher than before intervention (77.92% vs 49.78%; 76.47% vs 37.72%) (P＜0.05). Hand sanitizer usage increased from  2.14mL/ bedday to 4.63 mL/ bedday , HAI rate decreased from 1.97% to 1.54% (P＜0.05).ConclusionBundle hand hygiene intervention can improve HCWs’ knowledge awareness, compliance and execution rate of hand hygiene, and effectively reduce HAI rate.