ObjectiveTo investigate the status of disinfection and sterilization quality of different levels of medical institutes in Xi’an, and improve the management level of disinfection and sterilization of medical institutes. MethodsDisinfection and sterilization efficacy of 69 medical institutes were monitored and evaluated according to Standardization for Disinfection Techniques (2002 edition) and Sanitary Standards for Hospital Disinfection (GB15982-1995). ResultsFrom 2011 to 2013, 2 224 specimens were tested, 1 766 (79.41%) were qualified．The qualified rate of tertiary medical institutes was higher than second and below medical institutes （83.67％ vs 77.50％,P=0.001）， the qualified rate between public medical institutes and private medical institutes was not statistically different （79.64％ vs 78.20％, P=0.532）, the qualified rate of comprehensive medical institutes was higher than specialized medical institutes （80.18％ vs 74.92％,P=0.030）. Of different detected specimens, the qualified rates of disinfectant in use and pressure steam sterilizers （including test pack and process challenge device） were higher （98.46%,100.00%,and 98.06% respectively），while the hands of health care workers (HCWs) and glutaraldehyde were lower（58.48% and 43.28% respectively）．ConclusionDisinfection and sterilization quality in different medical institutes in Xi’an is different, and different disinfected and sterilized objects are also varied ．It is necessary to intensify the management of hand hygiene of HCWs and concentration of glutaraldehyde.