ObjectiveTo evaluate the influence of the placement of instant hand disinfectant in compliance and qualified rate of hand hygiene of clinicians. MethodsHand hygiene compliance of clinicians in departments of gynecology, obstetrics, and general surgery were observed after the placement of instant hand disinfectant at the doorway of wards, hand specimens were taken to check hand hygiene effectiveness. ResultsBefore and after placement of instant hand disinfectant at the doorway of wards of above departments, hand hygiene behaviors of 300 persontimes were observed. Hand hygiene compliance rate among clinicians in department of gynecology, obstetrics, and general surgery increased from 32.33% to 74.33%, 33.67% to 85.67%, and  26.33% to 64.00%，respectively（all P＜0. 001）. Each department selected 98 hand hygiene specimens, the qualified rate was 98.98%,100.00% and 94.90% respectively.  ConclusionThe placement of instant hand disinfectant at the doorway of each ward can effectively improve clinicians’ hand hygiene compliance and hand hygiene effectiveness.