ObjectiveTo analyze antimicrobial use in the different grades of hospitals within 3 years after the carrying out of rectification activities of antimicrobial use, and provide basis for making continuous improvement program of antimicrobial management. MethodsRetrospective analysis was performed, outpatient and emergency prescriptions and medical records of discharged patients (100 were type Ⅰ incision surgery) were selected from 5 tertiary hospitals and 5 secondary hospitals within 3 months, antimicrobial use and management were investigated. Results200 outpatient prescriptions, 100 emergency prescriptions, and 200 medical records of discharged patients were selected from each hospital. In tertiary and secondary hospitals, antimicrobial usage rate in outpatient prescriptions was 17.40% and 18.00% respectively,  emergency prescriptions was 31.60% and 40.10% respectively; among inpatients,antimicrobial usage density（AUD）was (28.13±8.04) and (49.67±11.63) respectively, and antimicrobial usage rate was 41.30% and 56.20% respectively; pathogenic detection rate of antimicrobial therapeutic cases was 45.70% and 22.70% respectively, therapeutic cases with limit level of antimicrobials was 61.50% and 27.40% respectively; antimicrobial usage rate in type I incision surgery was 20.00% and  40.40% respectively；The rational rate of antimicrobial types was 94.60% and  59.20% respectively, rational rate of opportunity of antimicrobial use was 95.20% and 48.20% respectively；combination rate was 8.80% and 36.60% respectively, and rational rate of treatment course was 84.00% and 52.80% respectively. ConclusionIn tertiary hospitals, antimicrobial use after 3 years rectification of antimicrobial use conform to the antimicrobial management requirement of Ministry of Health, the overall quality index is better than secondary hospitals, management of antimicrobial in secondary hospitals use should be improved.