ObjectiveTo evaluate the comprehensive medical goal appraisal system on hand hygiene compliance rate of health care workers(HCWs). MethodsComprehensive medical goal appraisal system was adopted to intervene hand hygiene compliance rate of HCWs  in a comprehensive hospital , hand hygiene compliance rates of HCWs and consumption of instant hand sanitizer per bedday before (December 2012) and after intervention (January 2013-June 2014) were compared. ResultsHand hygiene compliance rate after intervention was higher than before intervention (85.17% ［18 208/21 379］vs 39.92%［853/2 137］)，hand hygiene compliance rate enhanced by 113.35%（χ2=2 590.81，P＜0.001）.Hand hygiene compliance rates of HCWs of different departments, different occupations and different hand hygiene moments were all higher than before intervention (all P＜0.001）; after intervention , hand hygiene compliance rate revealed a increased tendency, and has maintained high since October 2013 (>90%), consumption of instant hand sanitizer before and after intervention was 7.24 mL/ bedday（4 200 L/579 841 bedday） and 10.54 mL/bedday（9 323.5L/884 489 bedday）respectively, the consumption after intervention increased by 45.58% compared with that before intervention.  ConclusionComprehensive medical goal appraisal can effectively enhance hand hygiene compliance rate , and maintains at a high level; the measure can affect hand hygiene behavior of HCWs by hawthorne effect, and is an effective and longterm measure to improve hand hygiene compliance of HCWs.