ObjectiveTo explore the risk factors for urosepsis  after minimally invasive percutaneous nephrolithotomy(mPCNL). MethodsClinical data of 1 022 patients undergoing mPCNL were collected, risk factors for urosepsis  after mPCNL were analyzed．ResultsOf 1 022 patients, 62 (6.07%) developed urosepsis. All patients recovered and discharged after early diagnosis and active treatment. Logistic regression analysis showed that age（OR95%CI：1.25-3.92）,blood routine examination （OR95%CI：1.25-14.00）,urine routine examination（OR95%CI：2.67-10.02）and  volume of  intraoperative fluid perfusion（OR95%CI： 1.37-4.18）were closely related to urosepsis following mPCNL. ConclusionAge（≥60 years）and  intraoperative perfusion fluid volume (≥30L）during operation are the risk factors for urosepsis following mPCNL, The recognition of the related risk factors is helpful for the prevention and timely treatment of urosepsis following mPCNL.