ObjectiveTo investigate the distribution and antimicrobial resistance of pathogens isolated from surgical patients with infection. MethodsDistribution and antimicrobial resistance of 1 208 pathogens isolated from surgical patients with infection from January 2013 to January 2014 were analyzed retrospectively. ResultsOf 1 208 pathogenic isolates, gramnegative bacteria, grampositive bacteria and fungi accounted for 64.57%（n=780），24.92%（n=301） and 10.51%（n=127） respectively. The main specimens were sputum (44.78%), urine (21.11%),blood(11. 51%), and pus（10.26%）.Antimicrobial suscept ibility testing results showed that the producing rate of extendedspectrum βlactamases (ESBLs)  of Escherichia coli and Klebsiella pneumoniae was 62.60% and 33.61% respectively, resistant rate to imipenem was 0.76% and 15.57%, respectively. The resistant rate of Pseudomonas aeruginosa and Acinetobacter baumannii to imipenem was 38.93% and 75.80% respectively. Methicillinresistant Staphylococcus aureus and methicillinresistant coagulase negative Staphylococcus was 71.68% and 87.93% respectively. ConclusionThe major pathogens isolated from surgical patients with infection are gramnegative bacteria, the main infection sites are respiratory tract and urinary tract in this hospital; multidrug resistance is serious, especially carbapenem resistance, which should be paid attention.