ObjectiveTo understand the distribution and antimicrobial resistance of pathogens isolated from bone and joint infection sites  of  orthopedic patients, so as to provide reference for  prevention and therapy of bone and joint infection. Methods114 specimens from bone and joint infection sites of orthopedic patients between June 2010 and October 2013 were surveyed, pathogens and antimicrobial susceptibility of pathogens were analyzed.ResultsA total of 145 pathogenic isolates were obtained, 62.07%(n=90) of which were gramnegative bacteria,34.48%(n=50) were grampositive bacteria, 2.07%(n=3) were Candida spp., and 1.38%(n=2) were anaerobic bacteria. The top 4 pathogens were Pseudomonas aeruginosa (20.69%), Staphylococcus aureus (12.41%), Escherichia coli (11.03 %), and Enterobacter cloacae (9.65%).These pathogens presented remarkable resistance. The resistant rate of Pseudomonas aeruginosa to ceftazidime was up to 40.00%; the resistant rates of Escherichia coli to compound sulfamethoxazole, ciprofloxacin and levofloxacin were all 100.00%.ConclusionGramnegative bacteria are the major pathogens in orthopedic ward, the isolated strains are resistant to multiple antimicrobial agents.