ObjectiveTo investigate the distribution and antimicrobial resistance of clinically isolated pathogens from geriatrics department of a hospital in 2013, so as to provide reference for clinical antimicrobial use．MethodsDistribution and antimicrobial resistance of pathogens isolated from patients in a geriatrics department between January and December 2013 were analyzed statistically. ResultsOf 1 896 pathogenic strains, 1 289(67.99%) were gramnegative bacteria, 439 (26.00%）were grampositive bacteria, and 114(6.01%) were fungi; the top 4 isolated pathogens were Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli. Extendedspectrum βlactamase (ESBL)producing Escherichia coli and Klebsiella pneumoniae accounted for  53.26% and 31.10% of Escherichia coli and Klebsiella pneumoniae respectively; Of Staphylococcus aureus and Staphylococcus epidermidis strains,  methicillinresistant isolates (MRSA and MRSE) accounted for 22.47% and  80.00%, respectively, of Enterococcus strains, vancomycinresistant isolates (VRE) was 3.10%,  Klebsiella pneumoniae  and Enterobacteriaceae were highly sensitive to imipenem, meropenem and ertapenem. Resistant rate of Acinetobacter baumannii to imipenem and meropenem was 79.48% and 80.35% respectively，Pseudomonas aeruginosa had the lowest resistant rate to amikacin (10.70%).Grampositive bacteria were highly sensitive to vancomycin and linezolid. ConclusionThe major pathogens isolated from the elderly patients are gramnegative bacteria, and antimicrobial resistance is serious, surveillance of antimicrobial resistance is important for rational use of antimicrobial agents and control of pathogen resistance.