ObjectiveTo compare whether there is a difference in medical cost of intensive care unit(ICU) patients with catheterassociated infection (CAI) between before and after targeted intervention. MethodsCAI in ICU patients in 2010(preintervention group) and 2013 (postintervention group) were investigated by retrospective survey, hospitalization cost of two groups of patients before and after intervention was compared.  ResultsThe morbidity and mortality in patients with CAI  both decreased significantly after intervention, morbidity of healthcareassociated infection(HAI) decreased from 13.47% in 2010 to 4.41% in 2013, mortality decreased from 10.36% in 2010 to 2.2% in 2103. Total hospitalization cost, blood transfusion cost, and cost of special material before and after the implementation of targeted intervention all significantly different (all P<0.05), the difference of procalcitonin and antimicrobial agents cost were also significant(all P<0.05). ConclusionMedical cost in ICU patients with CAI decreased after intervention.