ObjectiveTo analyze the distribution and antimicrobial resistance of pathogens isolated from department of hematology during the past three years.MethodsPathogenic strains isolated from patients hospitalized in a hematology department between January 2011 and December 2013  were collected, antimicrobial susceptibility testing was performed by KirbyBauer disk diffusion method or automatic system, antimicrobial susceptibility testing results were judged according to American Clinical and Laboratory Standards Institute 2011, data were analyzed by WHONET 56 software.ResultsA total of 462 clinical isolates were collected in 2011—2013, including 161 grampositive cocci isolates, 279 gramnegative bacilli, and 22 fungi. Of Staphylococcus spp, detection rate of methicillinresistant coagulase negative Staphylococcus（MRCNS）and methicillinresistant Staphylococcus aureus (MRSA) was 81.37％ and  62.50％respectively. The resistant rate of Staphylococcus spp. and Enterococcus spp. to linezolid was 1.69% and 3.57% respectively, resistant rate of Staphylococcus spp. to teicoplanin was 3.39%, vancomycinresistant grampositive coccus was not found.  Enterobacteriaceae strains Escherichia coli and Klebsiella pneumoniae were highly susceptible to carbapenems, the sensitivity rates were 97.56%—98.88%; while nonfermentative gramnegative bacilli Pseudomonas aeruginosa and Acinetobacter baumannii  strains were obviously resistant to carbapenems,the resistance rates were 38.71%—64.00%.ConclusionAntimicrobial resistance of major pathogenic strains from hematology department is high, antimicrobial agents should be used according to pathogenic distribution characteristics and antimicrobial susceptibility testing results, healthcareassociated infection control should be strengthened to reduce antimicrobial resistant rate