ObjectiveTo  investigate department distribution and antimicrobial resistance of mucoid Klebsiella pneumoniae(K. pneumoniae),so as to provide reference  for rational antimicrobial use and control of healthcareassociated infction.Methods80  K.pneumoniae isolates from clinic in JanuaryDecember 2013 were collected, mucoid  strains were selected, bacterial identification and antimicrobial susceptibility testing were performed by VITEK2 Compact identification system.ResultsA total of 28 mucoid K.pneumoniae isolates were obtained,  strains were mainly from respiratory specimens, the main departments were neurosurgical department. Susceptibility rates of mucoid K. pneumoniae to imipenem, piperacillin / tazobactam, ceftazidime,ciprofloxacin, and amikacin was 96.43%,96.43%,89.29%,92.86%, and 96.43%, respectively,nonmucoid K. pneumoniae  to above antimicrobial agents was 78.29%,78.29%,69.08%,72.37%, and 78.29% respectively.ConclusionAntimicrobial susceptibility rates of mucoid  K. pneumoniae are significantly higher than nonmucoid strains in vitro, biofilm should be considered in clinical therapy, antimicrobial agents should be chosen rationally.