ObjectiveTo understand healthcareassociated infection(HAI) in a maternal and child health care hospital, so as to provide scientific evidences for further targeted surveillance.MethodsA crosssectional survey was performed by bedside visiting and medical record reviewing.ResultsOf 768 hospitalized patients, 9(1.18%) had HAI, the top 3 highest prevalence rates were found in obstetrical intensive care unit (9.09%), neonatal intensive care unit (5.80%) and gynecological department II(2.22%). Antimicrobial usage rate was 30.34%(n=233), 134 of which (57.51%) were prophylactic use, 165 were monotherapy(70.82%). A total of 5 pathogenic bacteria were isolated, the number of Streptococcus agalactiae,Klebsiella pneumonia, Enterococcus faecalis, and Staphylococcus saprophyticus was 2,1,1, and 1 respectively, except Streptococcus agalactiae, the other 3 strains were multidrugresistant organisms(MDROs).ConclusionSurveillance on MDRO infection should be paid much attention, the occurrence of MDRO infection should be reduced through targeted and bundle intervention.