ObjectiveTo investigate hand hygiene status of health care workers (HCWs) in a hospital, and provide guides for improving hand hygiene compliance．MethodsHand hygiene intervention was adopted in a hospital between 2012 and 2014, hand hygiene compliance among HCWs and healthcareassociated infection in patients between August 1 and August 31 of each year were investigated and analyzed statistically. ResultsIn 2102-2014, hand hygiene compliance rate of HCWs was 59.55%，62.13%,and 65.16% respectively, which showed a increased trend (χ2=10.018, P=0.002), HAI rate was 2.13%，1.48% and 1.06% respectively, which showed a decreased trend (χ2=82.377, P<0.001); hand hygiene compliance rate of doctors was lower than nurses  （57.97% vs 65.97%）; Of different hand hygiene moments, hand hygiene compliance rate was the lowest before touching a patient（41.32%）, and highest after body fluid exposure （76.47%）. ConclusionComprehensive hand hygiene intervention in this hospital has achieved preliminary results, improvement of HCWs’ hand compliance is helpful for preventing HAI.