ObjectiveTo investigate the contamination of carbapenemresistant Acinetobacter baumannii (CRAB) from object surface of key departments in a hospital, and identify whether these CRAB were homologous.MethodsEnvironmental hygienic monitoring in intensive care unit (ICU), emergency intensive care unit(EICU), hemodialysis room and operating room was conducted. Acinetobacter baumannii (A. baumannii) isolated from ICU and EICU environmental specimens were amplified  and typed by enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERICPCR).ResultsExcept hand hygiene of health care workers in EICU was qualified, bacterial count of object surface of ICU and EICU were all unqualified; detection results of specimens from hemodialysis room and operating room were all qualified. A total of 53 specimens were taken from object surface of ICU and EICU, 7 (13.21%) A. baumannii isolates were isolated, and all were CRAB isolates, 6 of which were of the same genotype and were identical with  A.baumannii from  patients’ sputum.ConclusionCRAB isolated from object surface in key departments is homologous, cleaning and disinfection of environmental object surface should be intensified to reduce the occurrence of healthcareassociated infection.