ObjectiveTo evaluate the occurrence of multidrugresistant organism (MDRO) infection in a general hospital, and take effective comprehensive intervention measures, so as to reduce MDRO infection.MethodsTargeted monitoring on MDROs was performed from October 2012 to December 2013, comprehensive intervention measures were conducted, the occurrence of MDRO infection before and after intervention was compared.ResultsA total of 62 384 patients were investigated, 17.91%（n=11 176）were sent specimens for pathogen culture, 606 times of culture for patients’ specimens  were detected MDROs,  292(0.47%) cases were MDRO healthcareassociated infection(HAI)，314 were communityacquired infection or colonization. MDRO infection case rate decreased from 0.65%（75/11 603） before intervention to 0.26%（36/13 875）after intervention (χ2=21.08，P＜0.05）.ConclusionComprehensive intervention measures can effectively reduce the occurrence of MDRO HAI.