ObjectiveTo construct a questionnaire for patients to participate  in hand hygiene promotion in medical institutions, and provide a measuring tool for patients to participate in hand hygiene promotion.MethodsA questionnaire was constructed by methods of literature research, expert panel discussion, preinvestigation, and Delphi.ResultsThis questionnaire was designed respectively for patients and health care workers, each contained 20 questions, involving hand hygiene perception, willingness to participate in promoting hand hygiene, and participation method, et al.ConclusionA  questionnaire for patients to participate hand hygiene promotion in medical institutions is preliminary constructed, which can be used for evaluating patients’ and HCWs’ willingness and relevant influencing factors of patients’ participation in hand hygiene promotion in medical institutions.