ObjectiveTo train health care workers (HCWs) by method of conventional training plus survey of hand contamination status, the influence of two kinds of methods in hand hygiene compliance of HCWs was evaluated. MethodsFrom November 2013 to April 2014, all HCWs in a cardiovascular internal medicine department were as trained subjects, they were divided into two groups (trial group and control group).Hand hygiene compliance status was investigated 1 month before training. In the  first month after training, conventional training method was adopted by both groups， from the second to fifth month, conventional training plus hand contamination survey was adopted by trial group, hand hygiene compliance between two groups were compared.ResultsHand hygiene compliance rates of trial group and  control group was 42.63% (107/251) and 41.80% (102/244) respectively before training, there was no significant difference（P＞0.05）.In the first and second month after training， hand hygiene compliance rate of  trial group was 55.70% (132/237) and 63.11% (154/244) respectively,control group was 56.52% (130/230) and 62.61% (149/238) respectively， compared with pretraining， the differences were significant (both P＜0.05), but the difference was not significant between two groups(P＞0.05); From the third to fifth month, hand hygiene compliance rates of trial group was 60.73%（150/247）,61.44%（145/236）,and 61.22%（150/245）respectively, control group was 51.68%（123/238）,51.02%（125/245）,and 52.32%（124/237）respectively, there was significant difference between two groups(P＜0.05).ConclusionConventional training combined with survey of hand contamination status can promote hand hygiene compliance of HCWs.