ObjectiveTo analyze the surveillance result of antimicrobial resistance of nonfermentative gramnegative bacilli(NFGNB) from a hospital in  2011—2013, and guide rational antimicrobial use. MethodsNFGNB were identified by US BD Phoenix100 automated microbial identification system, and antimicrobial susceptibility analysis were tested by microdilution method.ResultsA total of 3 138 NFGNB were isolated in 20112013, the detection rate of Pseudomonas aeruginosa (P. aeruginosa), Acinetobacter baumannii(A. baumannii), Stenotrophomonas maltophilia (S. maltophilia), and Burkholderia cepacia was 55.64% (n=1 746），30.21% (n=948），12.68% (n=398），and 1.47% (n=46）respectively. Respiratory specimens was the main specimen source(56.53%), strains were mainly isolated from patients in department of critical care medicine（26.48%）; resistant rates of P. aeruginosa and A. baumannii to carbapenems, βlactamase inhibitors, and aminoglycosides changed every year， but did not increase year by year, and declined in 2013; resistant rate of P. aeruginosa to cefepime, aztreonam, and moxifloxacin were all high(resistant to moxifloxacin was ＞90%);  resistant rates of  A.baumannii to most antimicrobial agents were high; resistant rate of S. maltophilia to compound sulfamethoxazole was relatively low.ConclusionAntimicrobial resistant rates of NFGNB changed each year, A. baumannii has a higher resistant rates to most antimicrobial agents, clinicians should use antimicrobial agents according to antimicrobial susceptibility testing result