ObjectiveTo analyze antimicrobial resistance of hospitalassociated methicillinresistant Staphylococcus aureus(HAMRSA)  and communityassociated methicillinresistant Staphylococcus aureus (CAMRSA), and provide reference for clinical treatment and  rational antimicrobial use.MethodsFrom May 2013 to June 2014, Staphylococcus aureus in a hospital were collected and analyzed， strains were identified and performed antimicrobial susceptibility testing by using VITEK 2 Compact system， diagnosis of HAMRSA and CAMRSA were confirmed in  combined with clinical symptoms.ResultsA total of 84 MRSA isolates were isolated (61 were HAMRSA strains, 23 were CAMRSA).Resistant rates of HAMRSA and CAMRSA to penicillin G and oxacillin were both 100.00%; to  ampicillin/sulbactam  was 100.00% and 95.65% respectively; to compound sulfamethoxazole was 39.34% and 34.78% respectively.  Antimicrobial resistant rates of HAMRSA to gentamicin, tetracycline, erythromycin, clindamycin, levofloxacin，ciprofloxacin, moxifloxacin, nitrofurantoin, and  rifampicin were all higher than CAMRSA, the difference were significant(all P＜0.001).ConclusionAntimicrobial resistance of HAMRSA and CAMRSA are all serious, monitor should be intensified, antimicrobial use should be chosen according to antimicrobial susceptibility testing result.