ObjectiveTo investigate the current status of healthcareassociated infection(HAI) and antimicrobial usage, so as to  provide a scientific basis for improving the management of HAI. MethodsA crosssectional survey was conducted by combination of  bedside visiting and medical records reviewing,  HAI were investigated among all  hospitalized patients  between 0：00 and 24：00 on August 21,2014.ResultsA total of 2 216 patients were investigated, the prevalence rate of HAI was 4.83％, the case infection rate was 5.14％; the main infection site was lower respiratory tract(63.16％), antimicrobial usage rate was 39.71％, the proportion of prophylactic  and therapeutic use of antimicrobial agents was 32.27% and 61.71% respectively.596 patients received therapeutic antimicrobial use, specimen detection rate was 56.21%（n=335）, the detection rate of pathogens was 15.52%（n=52）. The major detected bacteria were Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, and Stenotrophomonas maltophilia.ConclusionHAI prevalence survey is helpful for realizing the occurrence of HAI, respiratory tract  is the main infection site, gramnegative bacteria is the major pathogen, management of prophylactic use of antimicrobial agents is the focus of HAI management.