ObjectiveTo improve health care workers’ （HCWs） infection prevention and control consciousness through carrying out catheterassociated urinary tract infection(CAUTI) prevention and control project,and implement prevention and control measures in practical work.MethodsDiagnostic criteria of CAUTI issued by the U.S. Centers for Disease Prevention and Control / National Healthcare Safety Network were adopted, from October 2013 to September 2014, monitoring was conducted on CAUTI in patients with indwelling urinary catheters for ＞2 consecutive days at different types of ICUs in hospitals participated in the project .ResultsA total of 14 124 CAUTI cases were collected, 410 patients developed  CAUTI, urinary catheter usage rate was 79.33%(203 114/256 042), incidence of CAUTI was 2.02‰(410/203 114), urine specimen detection rate was 46.52%(6 570 /14 124). The detection rate of urine specimens increased from 37.24% (the fourth quarter of 2013) to 49.76% (the third quarter of 2014). Of prevention measures, the difference in the indication of catheterization was significantly different(P<0.01).ConclusionThrough  the development of competence building project of healthcareassociated infection prevention and control, prevention and control of CAUTI  has achieved good intervention effect.