ObjectiveTo evaluate the effectiveness of comprehensive intervention measures in reducing the incidences of multidrugresistant organism (MDRO) healthcareassociated infection(HAI) in intensive care units (ICUs). MethodsPatients admitted to 6 adult ICUs between January and December 2014 were chosen, and divided into two groups, patients admitted to ICUs between January and June  2014 (without taking special intervention measures) were as control group, between July and December 2014 (taking comprehensive MORO prevention and control measures) were as comprehensive intervention group. Incidences of MDRO HAI of two groups were compared.ResultsOf 1 030 patients in comprehensive intervention group, 391 were detected MDROs,105 developed  MORO HAI, incidence of HAI was 7.63/1 000 patientdays(105/13 753); of 1 230 patients in control group, 596 were detected MDROs, 133 developed  MORO HAI, incidence of HAI was 27.12/1 000 patientdays( 133/4 905),incidence of HAI in comprehensive intervention group was significantly  lower than control group(RR，0.28［95%CI，0.220.37］，P＜0.001）.ConclusionComprehensive intervention measures can effectively reduce the incidence of MORO HAI in ICUs.