阿奇霉素上调生物被膜阳性铜绿假单胞菌Ⅰ类整合酶基因的表达
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曾邦伟

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R378.99+1

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福建省卫生厅青年基金(2012-2-41);中华医院感染控制研究基金(ZHYG2014-0010)


ZENG Bangwei1, CHEN Jiansen1,PAN Yuhong1,WU Xiangyan2
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    摘要:

    目的探讨阿奇霉素对生物被膜(BF)阳性铜绿假单胞菌(PA)Ⅰ类整合酶基因 (intI1)mRNA表达量的影响。方法对某院临床分离的10株PA进行intI1基因检测,选择其中1株BF+intI1基因阳性的PA进行培养,并设空白对照组和阿奇霉素处理组(按阿奇霉素浓度不同分为3个浓度组,分别为16、32、64 mg/L组),重复试验5次,采用荧光定量聚合酶链式反应(RT-PCR)检测其intI1 mRNA的表达情况。结果16 mg/L阿奇霉素组、32 mg/L阿奇霉素组、64 mg/L阿奇霉素组和对照组intI1 mRNA相对表达量分别为(1.15±0.04)、(12.47±3.10)、(19.71±0.78)和(1.00±0.00),各组间比较,差异有统计学意义(F=163.82,P<0.001);组间两两比较,除低浓度阿奇霉素组(16 mg/L)与对照组比较,差异无统计学意义(P>0.05)外,其他各组间比较,差异均有统计学意义(P<0.05),阿奇霉素组intI1 mRNA表达量随培养液中阿奇霉素浓度升高而升高。结论在阿奇霉素压力下BF阳性的PA,intI1表达有所上调,可提高耐药基因的捕获概率,促进耐药基因重组。

    Abstract:

    ObjectiveTo evaluate the  effect of azithromycin on class Ⅰ integronintegrase gene (intI1) mRNA expression in biofilmforming (BF) Pseudomonas aeruginosa(PA).MethodsintI1 of 10 PA strains isolated from a hospital were detected, 1 strain with positive BF+intI1 was selected for culture, blank control group and three azithromycin trial groups (divided according to 3 concentrations:16 mg/L, 32 mg/L, and 64 mg/L) were set, experiments were repeated 5 times, expression of intI1 mRNA were detected by RTPCR. ResultsRelative expression of intI1 mRNA in azithromycin groups of 16 mg/L, 32 mg/L,  64 mg/L, and control group were (1.15±0.04),(12.47±3.10),(19.71±0.78), and (1.00±0.00),respectively, there were significant difference among four groups(F=163.82,P<0.001);intI1 mRNA expression between 16mg/L azithromycin group and control group was not significantly different (P>0.05), but among other groups were significantly different (P<0.05), intI1 mRNA expression in azithromycin groups increased with the enhancing concentration of azithromycin in culture solution .ConclusionExpression of intI1 gene mRNA in BF PA can be upregulated by the present of azithromycin,which may improve the probability of drugresistant genes, and promote drugresistant gene recombination.

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曾邦伟,陈建森,潘玉红,等.阿奇霉素上调生物被膜阳性铜绿假单胞菌Ⅰ类整合酶基因的表达[J]. 中国感染控制杂志,2015,14(9):597-600. DOI:10.3969/j. issn.1671-9638.2015.09.005.
ZENG Bangwei, CHEN Jiansen, PAN Yuhong, et al. ZENG Bangwei1, CHEN Jiansen1,PAN Yuhong1,WU Xiangyan2[J]. Chin J Infect Control, 2015,14(9):597-600. DOI:10.3969/j. issn.1671-9638.2015.09.005.

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  • 收稿日期:2015-06-15
  • 最后修改日期:2015-07-22
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  • 在线发布日期: 2015-09-30
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