ObjectiveTo analyze the difference in constituent  and antimicrobial resistance of pathogens in communityassociated urinary tract infection(CAUTI)  and  healthcareassociated UTI(HAUTI) .  MethodsClinical data and microbial detection of urine specimens of 960 patients with UTI in a hospital between January 2013  and June 2014 were investigated retrospectively, difference in constituent  and antimicrobial resistance of pathogens were analyzed. Results403 cases were CAUTI, and 557 were HAUTI; pathogens in both CAUTI and  HAUTI were gramnegative bacteria, accounting for 78.16% and 66.97% respectively. Constituent of pathogens in CAUTI and HAUTI were significantly different（χ2 =21.68,P＜0.001）. Resistant rates of Escherichia coli to piperacillin / tazobactam, cefazolin, cefoperazone / sulbactam, aztreonam, meropenem, ertapenem, gentamicin, and compound sulfamethoxazole were all significantly different between CAUTI and HAUTI (all P<0.05); Except aztreonam, resistant rates of Escherichia coli in HAUTI to the other antimicrobial agents were all higher than CAUTI. Resistant rates of Enterococcus faecium in HAUTI to penicillin, ampicillin,  gentamicin, levofloxacin, ciprofloxacin, and moxifloxacin were all higher than CAUTI(all P<0.05). ConclusionConstituent and antimicrobial resistance of pathogens in CAUTI and HAUTI are different, proper antimicrobial agents should be chosen according to constituent features of pathogens and change in antimicrobial resistance, so as to prevent and reduce the emergence of drugresistant strains, and improve therapeutic effectiveness