ObjectiveTo investigate in vitro activity of antimicrobial agents against Enterococcus spp. isolated from clinic specimens in a hospital.Methods188 Enterococcus spp. isolates from specimens sent by clinic departments in June 2013July 2014 were identified and performed antimicrobial susceptibility testing.ResultsOf 188 Enterococcus spp. isolates, 119 were Enterococcus faecium(E. faecium), 60 were E. faecalis, and 9 were E. avium, these strains  were mainly isolated from urine (34.57%) and blood specimens(19.15% ).  No daptomycin and linezolidresistant strain was detected;resistant rates of E. faecium to vancomycin was 1.68%, to penicillin，ampicillin，high concentration gentamycin，erythromycin,and levofloxacin were all ＞ 70%; except tetracycline, resistant rates of E.faecalis to the other antimicrobial agents were all lower than E. faecium, resistant rates of E. faecalis to penicillin and ampicillin were 16.67% and 13.33%  respectively.ConclusionDaptomycin has high activity against Enterococcus spp. in this hospital.