ObjectiveTo understand the specimen sources, clinical characteristics, and antimicrobial resistance of Burkholderia cepacia(B. cepacia) isolated from infected patients  in intensive care unit(ICU), so as to provide reference for guiding rational use of antimicrobial agents.MethodsClinical data of patients with B. cepacia infection in an ICU between 2011 and 2014 were analyzed retrospectively,  antimicrobial resistance of  strains was analyzed.ResultsA total of 267 B. cepacia strains were isolated, the major specimen sources  were sputum （80.15%,n=214）,  blood（14.23%,n=38）,and urine（3.37%,n=9）. Antimicrobial susceptibility testing results revealed that B. cepacia had multiple resistance, and was naturally resistant to multiple clinically used  antimicrobial agents, such as ampicillin, cefazolin, ampicillin/sulbactam, nitrofurantoin, and cefuroxime,resistant rates  were all 100%; resistant rates to ceftazidime  and levofloxacin were 4.12%  and 3.00% respectively；resistant rate to compound sulfamethoxazole had increased tendency（χ2=5.885, P=0.015）.ConclusionIsolation of B. cepacia in  ICU increased year by year, antimicrobial resistance is serious, management and targeted monitoring of prevention and control of healthcareassociated infection should be strengthened， antimicrobial agents should be chosen according to antimicrobial susceptibility testing results.